顾颖,博士 GU Ying, Ph.D.
副教授,硕士生导师 Associate Professor
电话:+86-592-2187880
传真:+86-592-2181258
E-mail:guying@xmu.edu.cn
加拿大pc:简历 (Education and Work experience)
1996年,厦门大学生物学专业,学士学位;
2006年,厦门大学生化与分子生物学专业/细胞生物学专业,博士学位;
2006年,厦门大学生命科学加拿大pc助理教授、硕士生导师;
2013年,厦门大学生命科学加拿大pc副教授;
B.S. Biochemistry, Xiamen University, 2000
Ph.D. Biochemistry & Molecular Biology/Cell Biology, Xiamen University, 2006
Assistant Professor, School of Life Sciences, Xiamen University, 2006
Associate Professor, School of Life Sciences, Xiamen University, 2013
加拿大pc:研究领域 (Research Area)
1、抗病毒的单抗筛。豪孟赴P秃椭刈榭乖冉锌共《镜牡タ股秆。鏗IV中和单抗、识别免疫优势表位单抗等的筛选。
2、基于表位的抗原研究:利用基因工程重组技术表达获得含有功能表位的抗原或颗粒化抗原。
3、功能性表位研究:应用生物传感器、分析超离、微量热等方法研究抗原-抗体的相互作用,尝试解析免疫复合物的空间结构,鉴定功能性表位的特征。
4、Generation of monoclonal antibodies against different viral targets : based on functional screening by virus-infection cell model, recombinant antigen reactivity and et. al, focusing on HIV neutralizing monoclonal antibodes, esp. those with broad neutralizing activity
5、Identification and characterization of key epitopes on viral antigens: Design and preparation of recombinant antigens harbouring functional epitopes
6、Functional characterization of epitopes: epitope mapping, structural analysis and function interpretation by various methods including SPR-based assay, analytical ultracentrifugation, microcalorimetry, and structural determination of antibody-antigen complexes
加拿大pc:代表性论文(Selected Publications)(* Corresponding author)
1、Gu Y, Tang X, Zhang X, Song C, Zheng M, Wang K, Zhang J, Ng MH, Hew CL, Li S*, Xia N*, Sivaraman J*. Structural basis for the neutralization of hepatitis E virus by a cross-genotype antibody. Cell Res. 2015 May;25(5):604-20.
2、Wang D, Li Z, Xiao J, Wang J, Zhang L, Liu Y, Fan F, Xin L, Wei M, Kong Z, Yu H, Gu Y, Zhang J, Li S, Xia N. Identification of Broad-Genotype HPV L2 Neutralization Site for Pan-HPV Vaccine Development by a Cross-Neutralizing Antibody. PLoS One. 2015 Apr 23;10(4):e0123944.
3、Gu Y, Cao F, Wang L, Hou W, Zhang J, Hew CL, Li S*, Yuan YA*, Xia N*. Structure of a Novel Shoulder-to-Shoulder p24 Dimer in Complex with the Broad-Spectrum Antibody A10F9 and Its Implication in Capsid Assembly. PLoS One. 2013 Apr 19;8(4):e61314.
4、Yang C, Pan H, Wei M, Zhang X, Wang N, Gu Y, Du H, Zhang J,Li S*, Xia N*.Hepatitis Evirus capsid protein assembles in 4M urea in the presence of salts.Protein Sci. 2013 Mar;22(3):314-26.
5、Gu Y, Hou W, Xu C, Li S, Shih JW, Xia N*. The enhancement of RNAi against HIV in vitro and in vivo using H-2K(k) protein as a sorting method. J Virol Methods. 2012 Jun;182(1-2):9-17.
6、Shen L1,